DBASS

DBASS3 - Record Details

Gene ATM
(Ensembl)
Location of Aberrant 3' Splice Site
Exon
Phenotype Ataxia telangiectasia
(OMIM)
Distance between Authentic and Aberrant 3' Splice Site (nt)
+1
Mutation IVS29-1G>A Change in the Reading Frame
+1
Reference(s)

Eng et al. (2004) Nonclassical splicing mutations in the coding and noncoding regions of the ATM Gene: maximum entropy estimates of splice junction strengths. Hum. Mutat., 23, 67-76. Pub Med Article # 14695534

Nucleotide Sequence[a] tttgtgattt   tattgaaagt   atagtttttc   agtagaaaaa   tggtttttga   atttgggggt   tattaaaatc   taaattttca   ttttggaagt   tcactggtct   atgaacaaaa   ctttttaaaa   cgatgactgt   attttttccc   ttaactctgt   ta(g>a)G/A   TTTGGATCCT   GCTCCTAATC   CACCTCATTT   TCCATCGCAT   GTGATTAAAG   CAACATTTGC   CTATATCAGC   AATTGTCATA   AAACCAAGTT   AAAAAGCATT   TTAGAAATTC   TTTCCAAAAG   CCCTgtaagt  
Terminal Exon? No
Splice Site Strength[b]
Authentic Site
Aberrant Site
  Maximum Entropy Model
6.21
5.11
  First Order Markov Model
7.65
5.72
  Weight Matrix Model
8.65
5.53
Genomic Co-ordinates (hg38)
  Aberrant Splice Site

chr11:108288978/108288979

Mutation

chr11:108288976

Legend

  • [a] /, cryptic or de novo splice sites; > nucleotide substitutions; (), deletions; [], duplications or insertions. Intronic sequences are in lower case (in blue), exons are in upper case (in green)
  • [b] Shapiro and Senapathy matrix scores were calculated according to an algorithm published previously (1, 2). Maximum entropy and the first-order Markov and weight matrix models were computed as described (3).

References

  1. Shapiro, M.B. and Senapathy, P. (1987) RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res., 15, 7155-7174.
  2. Senapathy, P., Shapiro, M.B. and Harris, N.L. (1990) Splice junctions, branch point sites, and exons: sequence statistics, identification, and applications to genome project. Methods Enzymol., 183, 252-278.
  3. Yeo, G. and Burge, C.B. (2004) Maximum entropy modeling of short sequence motifs with applications to RNA splicing signals. J. Comput. Biol., 11, 377-394.